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You are here: Home Publications available as pdfs Aarnes, S.G., Bellemain, E., Eiken, H.G. & Wartiainen, I. 2009. Interlaboratory comparison of genetic profiles of brown bears from Sweden (Laboratoire d’Ecologie Alpine) and Norway (Bioforsk Svanhovd). Bioforsk Report Vol. 4 No. 133. Bioforsk, Svanhovd
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Aarnes, S.G., Bellemain, E., Eiken, H.G. & Wartiainen, I. 2009. Interlaboratory comparison of genetic profiles of brown bears from Sweden (Laboratoire d’Ecologie Alpine) and Norway (Bioforsk Svanhovd). Bioforsk Report Vol. 4 No. 133. Bioforsk, Svanhovd

by Eva Bellemain last modified 2011-01-30 21:27

Comparisons of individual DNA-profiles between different laboratories require that the data can be standardized. In this study, we compared DNA profiles of brown bears (Ursus arctos) from Sweden with DNA profiles of Norwegian brown bears. Brown bear samples from Sweden were analyzed at Laboratoire d’Ecologie Alpine (LECA) in France, while the samples collected in Norway were analyzed in the DNA laboratory at Bioforsk Svanhovd. In April 2008, DNA from 38 different bears were analyzed both at LECA in France and at Bioforsk Svanhovd in Norway, which allowed to estimate a first calibrations keys and normalise the data. Calibrations key calculated from this study were used, in 2009, on genotypes from 79 individuals from south-Norway to compare with the genotypes in the database for Swedish brown bears. The initial comparison gave no match. In this study, new calibration keys were determined in order to make the genotypes from Norwegian bears comparable with the whole Swedish bear genetic database. The comparison based on the new calibration key included 165 individuals from Norway (time period 2005-2009) and gave 42 matches with individuals from the database for Swedish brown bears (time period 2001-2009). Marker MU59 did not function well in this calibration and additional analyses are needed to sort out the problems with this marker.

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